TABLE 5.

Effects of sample type, ambient temperature, and diet on richness and diversity indices of the orders of the Firmicutes phylum in the fecal and rumen liquor samples of steers fed either alfalfa silage or sainfoin silage

Ambient temp (°C)Index value for sample typea
Rumen liquorFeces
RichnessDiversityRichnessDiversity
SMargalefShannonSimpsonSMargalefShannonSimpson
Alfalfa silage
    −11.152.24C1.360.8252.24C1.340.81
    −16.552.50BX1.46AX0.92AX52.49BX1.450.92
    −18.752.061.240.7252.06D1.250.73
    −19.151.931.15B0.6551.931.150.65
    −19.352.89AX1.54AX1.04AX52.89AX1.501.03
    −20.251.82D1.08B0.59B51.821.100.59
Total51.09E1.300.6751.09E1.290.67
SE50.0390.0810.0490.0390.0780.048
Sainfoin silage
    −11.152.23CY1.330.8152.24CY1.340.81
    −16.552.49B1.45A0.92A52.49B1.470.93
    −18.752.06Y1.240.7252.06DY1.240.72
    −19.151.93Y1.160.65Y51.93Y1.150.65
    −19.352.89A1.56A1.04A52.89A1.531.03
    −20.251.82EY1.08BY0.59BY51.82Y1.100.59
Total51.09FY1.300.6751.09EY1.290.67
SE0.0390.0810.0490.0390.0820.049
  • a S, number of orders present in the community. The richness and diversity indices shown above were calculated from the proportions of orders Bacillales and Lactobacillales in class Bacilli and orders Clostridiales and Erysipelotrichales in class Clostridia in the total bacterial populations in the samples. Values with different superscripts are statistically different (P < 0.05). ABC, the lack of a common letter for values in the same column denotes significance within the diet (P < 0.05); XYZ, the lack of a common letter for values in the same column denotes significance between the diets (P < 0.05). Index values do not represent numbers of operational taxonomic units; they are abstract values. Data presented are mean values for fecal and rumen liquor samples from two pens at six sampling times.