UniFrac and Analysis of Traits statistics on phylogenetic clustering of Entomoplasmatales strains from well-sampled arthropod groupsa

ComparisonbEnvironment (nc)UniFracAnalysis of Traits
12DistancedP valueeD statisticd,fP value
Differences between host taxa and strains from all remaining environments (entire phylogeny)Army ants (36)All others (87)0.8086≤0.0010.04<0.000001
Other ants (12)All others (111)0.78310.21200.0770.00004
Spiders, Araneae (6)All others (117)0.89880.0030.0340.00791
Moths and butterflies, Lepidoptera (10)All others (113)0.83210.0310.082<0.000001
Fruit flies, Drosophila (12)All others (111)0.8903≤0.0010.061<0.000001
Differences between army ant subfamilies (primary army ant clade)Aenictinae (14)Dorylinae (7)0.63110.006NAgNA
Dorylinae (7)Ecitoninae (15)0.45650.004NANA
Ecitoninae (15)Aenictinae (14)0.70180.310NANA
Differences between army ant genera (primary army ant clade)Aenictus (14)Dorylus (7)0.63310.013NANA
Dorylus (7)Eciton (7)0.6453≤0.001NANA
Eciton (7)Aenictus (14)0.4049≤0.001NANA
  • a n ≥ 6 host species.

  • b Analyses focused on bacteria from the entire phylogeny or only on those from a subset within the primary army ant clade (see the supplemental material [Fig. S2] for the phylogeny and more details on the analyses).

  • c Sample sizes in parentheses indicate the numbers of bacterial strains falling into each of the compared categories.

  • d Higher UniFrac distances and lower Analysis of Traits D statistics imply greater phylogenetic separation of bacteria from the two focal categories.

  • e Generated using UniFrac's “compare each pair” test.

  • f Analysis of Traits analysis could be performed only on the entire 16S rRNA phylogeny.

  • g NA, not analyzed.