TABLE 4.

Biomass and lipid yield of S. obliquus in PL alone and in combination with MSST and FP discharges under optimized conditionsa

Culture conditionBiomass (g liter−1)Lipid yield (mg liter−1)b
N 11 control1.1 ± 0.1a118.7 ± 1.8a (11)
PL (20 g liter−1)1.9 ± 0.2b957.4 ± 2.2b (51)
MSST+PL (15 g liter−1)2.0 ± 0.2b1,048.9 ± 7.7c (53)
FP+PL (15 g liter−1)1.8 ± 0.2b947.4 ± 5.7b (54)
  • a Optimized conditions were as follows: when the stationary-phase culture was transferred to the medium containing 0.04 g liter−1 nitrate, 0.03 g liter−1 phosphate, and 1.0 g liter−1 thiosulfate for 8 days, the lipid content was boosted from 11% to 58% on the basis of comparisons to dry cell weight (13). Values represent averages ± standard errors based on three independent determinations. Values followed by the same letter did not differ significantly, as determined by Duncan's multiple-range test (P < 0.05). A separate analysis was done for each column.

  • b Values in parentheses represent lipid content determined on the basis of percent dry cell weight.