Table 1.

Bacterial strains used in this studya

StrainRelevant characteristic(s)Source or reference
E. coli DH5αF Φ80lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rk, mk+) supE44 thi-1 gyrA96 relA1 phoA22
C. glutamicum WTWT strain ATCC 13032, biotin auxotrophicAmerican Type Culture Collection
C. glutamicum ΔaceE Δpqo ΔilvEC. glutamicum WT with deletion of aceE, pqo, and ilvE genes, encoding the E1p subunit of the pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase, and transaminase B, respectively28
C. glutamicum ΔaceE Δpqo ΔilvE ΔldhAC. glutamicum ΔaceE Δpqo ΔilvE with an additional deletion of the ldhA gene, encoding l-lactate dehydrogenaseThis work
C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA ΔmdhC. glutamicum ΔaceE Δpqo ΔilvE ΔldhA with an additional deletion of the mdh gene, encoding malate dehydrogenaseThis work
C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA ΔmalEC. glutamicum ΔaceE Δpqo ΔilvE ΔldhA with an additional deletion of the malE gene, encoding malic enzymeThis work
C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh ΔmalEC. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh with an additional deletion of the malE gene, encoding malic enzymeThis work
C. glutamicum Iso1C. glutamicum ΔaceE Δpqo ΔilvE(pJC4ilvBNCD)This work
C. glutamicum Iso2C. glutamicum ΔaceE Δpqo ΔilvE(pJC4ilvBNCD)(pBB1kivd-adh2)This work
C. glutamicum Iso3C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA(pJC4ilvBNCD) (pBB1kivd-adh2)This work
C. glutamicum Iso4C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh(pJC4ilvBNCD) (pBB1kivd-adh2)This work
C. glutamicum Iso5C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh(pJC4ilvBNCD-pntAB) (pBB1kivd-adh2)This work
C. glutamicum Iso6C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA(pJC4ilvBNCD)(pBB1kivd)This work
C. glutamicum Iso7C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh(pJC4ilvBNCD-pntAB)(pBB1kivd-adhA)This work
C. glutamicum Iso8C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA ΔmalE(pJC4ilvBNCD)(pBB1kivd-adh2)This work
C. glutamicum Iso9C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh ΔmalE(pJC4ilvBNCD-pntAB)(pBB1kivd-adhA)This work
  • a WT, wild type.