Table 1.

PCR primers used for sequencing reactionsa

PCR primer namePrimer sequence
RecA 01F-external5′-TGA(A/G)AA(A/G)CA(A/G)TT(C/T)GGTAAAGG-3′
RecA 02R-external5′-TC(A/G)CC(A/G/C/T)TT(A/G)TAGCT(A/G)TACC-3′
RecA 03F-internal5′-T(C/T)GG(G/C/T)GTGATGTT(C/T)GGTAACC-3′
RecA 04R-internal5′-GGGTTACC(A/G)AACATCAC(A/G/C)CC-3′
RpoA 01F-external5′-ATGCAGGGTTCTGT(A/G/T)ACAG-3′
RpoA 03R-external5′-G(A/C/T)GGCCA(A/G)TTTTC(A/C/T)A(A/G)(A/G)CGC-3′
RpoA 05F-internal5′-GCAGC(A/G/T)CGTGT(A/T)GA(A/G)CA(A/G)AG-3′
RpoA 06R-internal5′-CG(C/T)TG(C/T)TC(A/T)ACACG(A/C/T)GCTGC-3′
16S rRNA 519F5′-CAGCAGCCGCGGTAA-3′
16S rRNA 529R5′-CGCGGCTGCTGGCAC-3′
16S rRNA 907R5′-CCCCGTCAATTCCTTTGAGTTT-3′
16S rRNA 1099F5′-GCAACGAGCGCAACCC-3′
16S rRNA 1240R5′-CCATTGTAGCACGTGT-3′
  • a Primers for recA and rpoA are from the work of Thompson et al. (39), and primers for 16S rRNA gene sequencing are found in the work of Dang and Lovell (13).