Table 1.

Bacterial and archaeal diversity in incubated hard-coal samples amended with [13C]acetate or H2-13CO2a

Labeled substrate and closest cultured relativeSimilarity (%)Band letter in Fig. 2GenBank accession no.
[13C]acetate
    Bacteria
        Burkholderia cepacia100AFR838981
        Pseudoalteromonas sp.100BFR838963
        Clostridium sp.99CFR838991
        Pseudomonas stutzeri99DFR838972
        Desulfovibrio africanus100EFR838976
        Pelobacter acetylenicus95FFR838952
        Uncultured Geobacteraceae99GFR838962
    Archaea
        Methanosaeta sp.98aFR838958
        Uncultured Crenarchaeota98bFR838970
        Methanosarcina barkeri99cFR838992
        Methanosarcina sp.99dFR838971
H2-13CO2
    Bacteria
        Burkholderia cepacia100AFR838986
        Desulfovibrio alkaliphilus92BFR838983
        Clostridium sp.99CFR838998
        Desulfovibrio africanus100DFR838979
        Pelobacter acetylenicus95EFR838951
        Uncultured Geobacteraceae98FFR838960
        Xanthomonas sp.100GFR838954
    Archaea
        Methanosaeta sp.98aFR838957
        Uncultured Crenarchaeota99bFR838966
        Methanosarcina barkeri93cFR838997
  • a Detected by denaturing gradient gel electrophoresis of density-resolved DNA gradient fractions. The phylogenetic affiliations of the 16S rRNA genes from microbes that incorporated 13C-labeled substrates are shaded.