Table 2.

Bacterial and archaeal diversity in incubated mine timber samples amended with [13C]acetate and H2-13CO2a

Labeled substrate and closest cultured relativeSimilarity (%)Band letter in Fig. 3GenBank accession no.
[13C]acetate
    Bacteria
        Acholeplasma sp.95AFR838980
        Uncultured Bacteroidetes99BFR838984
        Clostridium sp.99CFR838974
        Burkholderia cepacia100DFR838994
        Hyphomicrobium sp.99EFR838953
        Hydrogenophaga sp.94FFR838955
        Desulfovibrio sp.95GFR838961
        Desulfovibrio africanus100HFR838982
        Halochromatium sp.92IFR838989
        Desulfuromonas acetexigens95JFR838965
        Uncultured Geobacteraceae96KFR838964
    Archaea
        Uncultured Crenarchaeota98aFR838968
        Uncultured Crenarchaeota98bFR838969
        Methanosaeta sp.98cFR838959
        Methanosarcina barkeri99dFR838995
        Methanosarcina barkeri93eFR838996
H2-13CO2
    Bacteria
        Acholeplasma sp.95AFR838990
        Uncultured Bacteroidetes99BFR838988
        Clostridium sp.99CFR838975
        Burkholderia cepacia99DFR838987
        Hyphomicrobium sp.99EFR838956
        Hydrogenophaga sp.94FFR838985
        Desulfovibrio sp.95GFR838973
        Desulfovibrio africanus95HFR838999
    Archaea
        Uncultured Crenarchaeota98aFR838967
        Methanosarcina sp.100bFR838977
        Methanosarcina sp.100cFR838978
        Methanosarcina barkeri99dFR838993
  • a Detected by denaturing gradient gel electrophoresis of density-resolved DNA gradient fractions. The phylogenetic affiliations of the 16S rRNA genes from microbes that incorporated 13C-labeled substrates are shaded.