Table 1.

Electron donors and acceptors supporting the growth of D. desulfuricans ND132a

SubstrateElectron acceptorGrowthb
Respiration
    LactateSulfate+++
    PyruvateSulfate+++
    MalateSulfate
    FumarateSulfate++
    SuccinateSulfate
    ProlineSulfate
    EtOHSulfate
    CysteineSulfate
    AlanineSulfate
    GlycinebetaineSulfate
    CholineSulfate
    Formate-acetateSulfate+
    LactateSulfite++
    PyruvateSulfite+
    Formate-acetateSulfite
    LactateNitrate
    LactateFumarate+
    PyruvateFumarate+++
    Formate-acetateFumarate+
    LactateFe(III)−*
    PyruvateFe(III)−*
    Pyruvate-acetateFe(III)−*
    AcetateFe(III)−*
Fermentation
    Lactate
    Pyruvate+
    Malate
    Fumarate+
    Succinate
    Proline
    EtOH
    Cysteine
    Alanine
    Glycinebetaine
    Choline
    Formate-acetate
  • a Growth in modified SRM (6) or MOY (71) medium, with potential organic substrates added at 30 to 60 mM concentrations, was tested. For formate, 20 mM Na-acetate was also added as the carbon source. Sulfate, sulfite, fumarate, and Fe(III) citrate (50 mM) were tested as potential electron acceptors. All media contained yeast extract. All tests were done in at least triplicate, and positive cultures were subcultured again in the same medium. Growth was measured once cells reached stationary phase.

  • b +++, OD660 ≥ 1.0; ++, OD660 ≥ 0.5; +, OD660 ≥ 0.1; *, no reduction of Fe(III).