Primer sets used for diagnostic and quantitative PCR
| Target organism(s) | Target gene | Primer name | Nucleotide sequence (5′→3′) | Product size (bp) | Annealing temp (°C) | Purpose | Source or reference |
|---|---|---|---|---|---|---|---|
| Stinkbug | Mitochondrial cytochrome oxidase I gene (COI) | LCO1490 | GGTCAACAAATCATAAAGATATTGG | 650 | 48 | Diagnostic PCR | 12 |
| HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | ||||||
| Burkholderia symbiont | 16S rRNA gene | Burk16SF | TTTTGGACAATGGGGGCAAC | 750 | 55 | Diagnostic PCR | 32 |
| Burk16SR | GCTCTTGCGTAGCAACTAAG | ||||||
| Burkholderia spp. | dnaA | BurkDnaA-F | GGCCTSGGCAAGACSCAYCT | 800 | 69 | Cloning | This study |
| BurkDnaA-R | GTSGTRTGRTCGCGGCCGCC | ||||||
| Burkholderia symbiont | dnaA | BSdnaA-F | AGCGCGAGATCAGACGGTCGTCGAT | 150 | 60 | Quantitative PCR | This study |
| BSdnaA-R | TCCGGCAAGTCGCGCACGCA |