Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicaSource or reference
Strains
    Escherichia coli
        DH5αF80d lacZ 15 recA1GIBCO-BRL
        C2110Nalr polA7
    Xanthomonas citri subsp. citri A44Cur9
    Xanthomonas alfalfae subsp. citrumelonis 1381CurThis study
    Xanthomonas perforans 91-118KanrThis study
Plasmids
    pLAFR3Tetr rlx+, RK2 replicon38
    pBluescript KS+/−Phagemid, pUC derivative, AmprStratagene
    pRK2073ColE1 replicon, Tra+ Mob+ Spr19
    pXccCu1Tetr Cur, ∼17-kb EcoRI-HindII fragment of X. citri subsp. citri A44 in pLAFR3This study
    pXccCu2Tetr Cur, 9.5-kb EcoRI-EcoRI fragment of pXccCu1This study
    pXacCu1Tetr Cur, ∼17-kb EcoRI-HindII fragment of X. alfalfae subsp. citrumelonis 1381 in pLAFR3This study
    pXacCu2Tetr Cur, 9.6-kb HindIII-EcoRI fragment of pXacCu1This study
  • a Nalr, nalidixic acid resistant; Kanr, kanamycin resistant; Tetr, tetracycline resistant; Ampr, ampicillin resistant; Spr, spectinomycin resistant; Cur, copper resistant.