Table 3.

Genes and operons of known function exhibiting increased transcription in early postexponential-phase cells of postsweep strain WN716

Category, subcategory, and gene or operonFunctionFold expression increase
Category 1. Cell envelope and cellular processes
    1.1 Cell wall
        lytBAmidase enhancer precursor (modifier protein of major autolysin)10
                1.2 Transport/binding proteins and lipoproteins
        amyCMultiple sugar transport system permease13
    1.8 Sporulation
        cotSSpore coat protein9
        cotVSpore coat protein (insoluble fraction)12
Category 2. Intermediary metabolism
    2.1 Metabolism of carbohydrates and related molecules
    2.1.1 Main glycolytic pathways
        pdhAPyruvate dehydrogenase E1 (alpha subunit)9
        pdhBPyruvate dehydrogenase E1 (beta subunit)10
        pdhCPyruvate dehydrogenase E2 subunit5
        pdhDPyruvate dehydrogenase E3 subunit10
    2.2 Metabolism of amino acids and related molecules
        asnHAsparagine synthetase (glutamine hydrolyzing)8
        nasBAssimilatory nitrate reductase, electron transfer subunit15
    2.3 Metabolism of nucleotides and nucleic acids
        purEPhosphoribosylaminoimidazole carboxylase I92
        purKPhosphoribosylaminoimidazole carboxylase II5
        purBAdenylosuccinate lyase9
        purCPhosphoribosylaminoimidazole succinocarboxamide synthetase6
        purSRequired for PurQL activity10
        purQPhosphoribosylformylglycinamidine synthase Ia
        purLPhosphoribosylformylglycinamidine synthase II7
        purFPhosphoribosylpyrophosphate amidotransferase3
        purMPhosphoribosylaminoimidazole synthetase2
        purNPhosphoribosylglycinamide formyltransferase1
        purHPhosphoribosylaminoimidazole carboxy formyl formyltransferase/inosine-monophosphate cyclohydrolase1
        purDPhosphoribosylglycinamide synthetase1
        pyrRTranscriptional attenuator of the pyrimidine operon/uracil phosphoribosyltransferase91
        pyrPUracil permease17
        pyrBAspartate carbamoyltransferase catalytic chain52
        pyrAACarbamoyl-phosphate synthase small chain70
        pyrABCarbamoyl-phosphate synthase large chain39
        pyrKDihydroorotate dehydrogenase (electron transfer subunit)24
        pyrDDihydroorotate dehydrogenase (catalytic subunit)24
        pyrFOrotidine 5′-phosphate decarboxylase19
        pyrEOrotate phosphoribosyltransferase19
    2.4 Metabolism of lipids
        napCarboxylesterase NA8
    2.6 Metabolism of phosphate
        phoBAlkaline phosphatase III8
Category 3. Information pathways
    3.2 DNA restriction/modification and repair
        dinGDNA damage-inducible ATP-dependent DNA helicase126
        mtbPModification methylase Bsu12
    3.5 RNA synthesis
        3.5.2 Transcription regulation
            ctsRTranscriptional repressor of class III stress genes12
            gerETranscriptional regulator required for expression of late spore coat genes8
            hrcATranscriptional repressor of class I heat shock genes20
            tnrATranscriptional pleiotropic regulator involved in global nitrogen regulation9
    3.7 Protein synthesis
        3.7.1 Ribosomal proteins
            rplLRibosomal protein L12 (BL9)9
    3.8 Protein folding
        groESClass I heat shock protein (chaperonin)12
        groELClass I heat shock protein (chaperonin)12
Category 4. Other functions
    4.1 Adaptation to atypical conditions
        clpCClass III stress response ATPase5
        clpEATP-dependent Clp protease (class III stress gene)44
        grpEHeat shock protein (activation of DnaK)5
        dnaKClass I heat shock protein, chaperone7
        gspAGeneral stress protein A8
        mcsAModulator of CtsR repression13
        mcsBModulator of CtsR repression8
        mrgAMetalloregulation DNA-binding stress protein16
    4.2 Detoxification
        katBCatalase 28
  • a –, signal not detected.