Table 1.

Sequences and properties of [FeFe]- and group 4 [NiFe]-hydrogenase primers

PrimerTarget groupSequenceaReferencePositionDeg.b
HydH1f[FeFe] (Bacteria)TIACITSITGYWSYCCIGSHTGGc47524d192
HydH3r[FeFe] (Bacteria)CAICCIYMIGGRCAISNCAT471126d64
NiFe-gF[NiFe] (Gammaproteobacteria)GAYCGIRTITGYGGIATYTGYGGThis study715e32
NiFe-gR[NiFe] (Gammaproteobacteria)GTRCAIGARTARCAIGGRTCThis study1585e16
NiFe-uniF[NiFe] (Bacteria/Archaea)GAIMGIRTITGYGGIATHTGYThis study715e48
NiFe-uniFb[NiFe] (Bacteria/Archaea)GARMGIGTITGYTCICTGTGYThis study715e16
NiFe-uniR[NiFe] (Bacteria/Archaea)GTRCAISWIWIRCAIGGRTCThis study1585e64
  • a I, inosine. IUPAC nomenclature was used for mixed bases (13).

  • b Deg., degeneracy, i.e., the number of combinations for the degenerate primers.

  • c The published primer was shortened by one nucleotide position at the 5′ end, as this position turned out to be variable in the [FeFe]-hydrogenase genes available from the public databases.

  • d The 5′ end in the nucleic acid sequence of the D. vulgaris [FeFe]-hydrogenase gene (GenBank accession no. AAS96246).

  • e The 5′ end in the nucleic acid sequence of the E. coli group 4 [NiFe]-hydrogenase gene (GenBank accession no. AAC75763).