Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Escherichia coli JM109recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+ lacIq lacZΔM15]Promega
Burkholderia contaminans
    MS14Wild-type strain13
    MS14MT15ambR2::Tn5 derivative of MS14; Kmr13
    MS14GG44ambR1::nptII derivative of MS14; Kmr14
    MS14MT24ocfH::Tn5 derivative of MS14; KmrThis study
    MS14GG78ocfJ::nptII derivative of MS14; KmrThis study
Plasmid/fosmid
    pSL604EZ-Tn5 carrying the ∼1-kb genomic DNA of MS14MT24; KmrThis study
    pBluescript II SKCloning vector; AprStratagene
    pBR325Cloning vector; Cmr Tcr Apr31
    pMLS7Expression vector of Burkholderia; Tpr21
    pGEM-T EasyCloning vector; AprPromega
    pBSL15Kanamycin resistance gene cassette; Kmr1
    pGG20pGEM-T Easy carrying 4-kb PCR product containing the ocfJ gene; AprThis study
    pGG21pGG20 with the nptII insertion in ocfJ at the BamHI site; Kmr AprThis study
    pGG22pBR325 carrying the 5-kb EcoRI fragment containing ocfJ gene disrupted by nptII; Cmr Tcr KmrThis study
    pGG23pGEM-T Easy carrying 4.5-kb PCR product containing the intact ocfJ gene; AprThis study
    pGG24pMLS7 carrying 4.5-kb HindIII fragment harboring the intact ocfJ gene; TprThis study
    4G5pCC1FOS carrying 40-kb genomic DNA of MS14; CmrThis study
  • a Kmr, kanamycin resistance; Apr, ampicillin resistance; Tpr, trimethoprim resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance.