Table 1.

Enzymatic activities associated with acetone and butanol production

EnzymeActivity (U/mg) in:
Strain N1-4Coculturea
CoA-transferase (acetate)29.72 ± 2.4327.43 ± 2.51
CoA-transferase (butyrate)28.64 ± 2.5529.36 ± 2.75
Acetoacetate decarboxylase140.81 ± 17.14168.08 ± 23.70
Butyraldehyde dehydrogenase1.24 ± 0.010.95 ± 0.13
Butanol dehydrogenase1.04 ± 0.070.90 ± 0.02
  • a Activities were normalized based on thiolase activity because crude extracts were prepared from cocultured strains N1-4 and C. thermocellum.