Table 1.

Apparent Kd values at 60°C of TreE and XylE2 for different sugars as measured by changes in intrinsic fluorescence upon ligand binding

ProteinSugarKd value at 60°C (μM)
TreETrehalose0.024
Sucrose0.300
Glucose56.78
XylE2Glucose0.059
Xylose0.042
l-Fucose1.436