Table 1.

Media used throughout this studya

MediumEnergy source (g liter−1)Goal
GCDMGlycerol (5)Screening of L. sakei strains for capacity to catabolize glycerol, in the absence of any background carbohydrates
mMRS1Glucose (1) and glycerol (1)Screening of L. sakei strains for capacity to co-catabolize glycerol
mMRS2Inosine (3)Screening of L. sakei strains for capacity to catabolize inosine
mMRS3Adenosine (3)Screening of L. sakei strains for capacity to catabolize adenosine
mMRS4Arginine (3) and inosine (3)Fermentation experiments with L. sakei CTC 494 to analyze arginine and inosine conversion at free pH and as a function of a constant pH; an anaerobic fermentation was performed in this medium to allow online measurement of carbon dioxide
mMRS5Arginine (3) and adenosine (3)Fermentation experiments with L. sakei CTC 494 to analyze arginine and adenosine conversion at free pH and as a function of a constant pH.
mMRS6Arginine (3) and ribose (3)Fermentation experiments with L. sakei CTC 494 to perform a kinetic analysis of conversion of arginine and ribose
mMRS7Arginine (3), inosine (3), and glucose (1)Fermentation experiments with L. sakei CTC 494 to unravel influence of glucose on catabolism of arginine and inosine
mMRS8Arginine (3), adenosine (3), and glucose (1)Fermentation experiments with L. sakei CTC 494 to unravel influence of glucose on catabolism of arginine and adenosine
  • a All modified MRS (mMRS) media were composed of customized MRS broth (8), whereby the glucose fraction was replaced by the appropriate energy sources. Glycerol-containing chemically defined medium (GCDM) was free of background carbohydrates (17). All chemicals were purchased from VWR International (Darmstadt, Germany).