Table 1.

Bacterial strains used in this study

StrainGenotypeReference or source
BE400E. coli strain K-12 substrain MG1655Laboratory collection
BE100E. coli BL21(DE3) RIL (Cmr) (for expression of rare RIL codons [from Stratagene])Laboratory collection
ZH99BE100, pBE522 (Kanr, Cmr)Laboratory collection
ZH100BE100, pBE522-eutE (Kanr, Cmr)This study
ZH103BE100, pBE522-His6-SeEutEThis study
ZH39BE400, pBE522 (Kanr)This study
ZH40BE400, pBE522-eutE (Kanr)This study
NA19BE400, adhE::FRTThis study
ZH58BE400, adhE::FRT, pLAC22 (Ampr)This study
ZH54BE400, adhE::FRT, pLAC22-adhE (Ampr)This study
ZH97BE400, adhE::FRT, pBE522 (Kanr)This study
ZH98BE400, adhE::FRT, pBE522-eutE (Kanr)This study
ZH44BE400, pta::FRTThis study
ZH57BE400, pta::FRT, pBE522 (Kanr)This study
ZH55BE400, pta::FRT, pBE522-pta (Kanr)This study
ZH94BE400, pta::FRT, pBE522-eutE (Kanr)This study
ZH46BE400, ackA::FRTThis study
ZH64BE400, ackA::FRT, pLAC22 (Ampr, Tetr)This study
ZH65BE400, ackA::FRT, pLAC22-ackA (Ampr)This study
ZH95BE400, ackA::FRT, pBE522 (Kanr)This study
ZH96BE400, ackA::FRT, pBE522-eutE (Kanr)This study
ZH28BE400, ldhA::FRTThis study
ZH41BE400, ldhA::FRT, pBE522 (Kanr)This study
ZH132BE400, ldhA::FRT, pBE522-ldhA (Kanr)This study
ZH42BE400, ldhA::FRT, pBE522-eutE (Kanr)This study
ZH71BE400, frdC::FRTThis study
ZH91BE400, frdC::FRT, pLAC22 (Ampr, Tetr)This study
ZH90BE400, frdC::FRT, pLAC22-frdC (Ampr)This study
ZH109BE400, frdC::FRT, pBE522 (Kanr)This study
ZH108BE400, frdC::FRT, pBE522-eutE (Kanr)This study
ZH30BE400, adhE::FRT, ackA-pta::FRTThis study
NA20BE400, adhE::FRT, ldhA::FRTLaboratory collection
ZH4BE400, adhE::FRT, ldhA::FRT, pBE522 (Kanr)This study
ZH3BE400, adhE::FRT, ldhA::FRT, pBE522-eutE (Kanr)This study
ZH111BE400, adhE::FRT, ackA-pta::FRT, pBE522 (Kanr)This study
ZH110BE400, adhE::FRT, ackA-pta::FRT, pBE522-eutE (Kanr)This study
ZH87BE400, adhE::FRT, ldhA::FRT, ackA-pta::FRT, frdC::FRT, pBE522 (Kanr)This study
ZH88BE400, adhE::FRT, ldhA::FRT, ackA-pta::FRT, frdC::FRT, pBE522-eutE (Kanr)This study
ZH134BE400, adhE::FRT, ldhA::FRT, ackA-pta::FRT, frdC::FRT, pBE522-eutE (Kanr), allyl alcohol resistant, isolate 1This study
ZH135BE400, adhE::FRT, ldhA::FRT, ackA-pta::FRT, frdC::FRT, pBE522-eutE (Kanr), allyl alcohol resistant, isolate 2This study
ZH136BE400, adhE::FRT, ldhA::FRT, ackA-pta::FRT, frdC::FRT, pBE522-eutE (Kanr), allyl alcohol resistant, isolate 3This study