Table 3.

Physiological parameters of recombinant P. putida KT2440 strains during growth on glucose and with different NADH oxidase levelsa

PlasmidDCPK (% [vol/vol])Specific growth rate (h−1)Biomass yield (g g−1)Specific carbon uptake rate [mmol (gCDW h)−1]In vitro Nox activity (U mgprotein−1)
pCOM1000.48 ± 0.020.53 ± 0.025.1 ± 0.20.01 ± 0.00
pCOM10-nox0.0020.43 ± 0.030.43 ± 0.025.6 ± 0.50.09 ± 0.03
pCOM10-nox0.050.39 ± 0.020.39 ± 0.025.5 ± 0.52.34 ± 0.22
  • a The values of specific growth rate, biomass yield, and specific carbon uptake rate were calculated during the exponential phase of growth, identified by the linear region in the ln(CDW)-versus-time plot.