Table 1.

Bacterial strains and plasmids used in this study

Species and strain or plasmidDescriptionReference or source
Strains
    L. monocytogenes
        568Wild type; serotype 1/2a; from shrimp-processing plant25
        32F11Tn917 mutant of 568; Ermr18
        568:ΔTreA568 deletion mutant; TreAThis study
        ΔTreA:pAM:TREOPpAM401:TreA complement of 568ΔT6P; Crmr Tets TreA+This study
        568/pAULAΔTreA568 carrying pAULA-ΔTreA with treA deletion insertThis study
    E. coli
        TCTOP:ΔTreATOP10 carrying pCR2.1 TOPO with 568 treA insert with deletionThis study
        TCTOP:TREOPTOP10 carrying pCR2.1 TOPO with intact 568 Tre operonThis study
        DH5αCloning host; F endA1 glnV44 thi-1 recA1 gyrA96 deoR nupG φ80lacZ ΔM15 Δ(lacZYA-argF)U169 hsdR17 (rK mK+) λ24
        XL10-GOLD KanrCloning host; endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F′[proAB lacIqZ ΔM15 Tn10(Tetr) Amy Tn5(Kanr)]Stratagene
        XLCVV1XL10-GOLD carrying pCVV1 expression vectorThis study
        TCE-pAULA:TreADH5α host carrying pAUL-A with treA deletion insertThis study
        TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 λInvitrogen
Plasmids
    pAUL-AGram-positive and Gram-negative shuttle vector; oriTS, LacZa′, Ermr10
    pAM401Gram-positive and Gram-negative shuttle vector; Crmr Tetr60
    pCR:ΔTreApCR2.1 TOPO with 568 treA insert with deletionThis study
    pAULA:ΔTreApAUL-A with truncated 568 treA gene; ErmrThis study
    pAM401:TREOPpAM401 with intact 568 treA gene; Crmr, Tets treA+This study
    pCVV1pCYB1-derived expression vector carrying B. subtilis treA; Tac promoter, Ampr56
    pCR2.1 TOPOColE1 Ampr Kanr lacZαInvitrogen