Table 1.

Deletion mutants constructed in this study, sequences of the respective genes, and putative structures targeted for deletion

StrainRelevant characteristic(s)Source or reference
Ea1189Wild type5
Ea1189ΔamsAmylovoran operon deletion mutant41
Ea1189ΔhofCDeletion of EAM_0729; putative type IV pilus structureThis study
Ea1189ΔhofDeletion of EAM_0729-0731; genes ppdD, hofB, and hofC of a putative type IV pilus structureThis study
Ea1189ΔfimDDeletion of EAM_0230; putative type I fimbria structureThis study
Ea1189ΔfimDeletion of EAM_0230-0237; genes fimAD, clpEF, fimAC, and faeHI of a putative type I fimbria structureThis study
Ea1189Δflg-3Deletion of EAM_2541-2562; genes flhCD, fliADST, and flgABCDEFGHIJKLMN of a putative flagellum structureThis study
Ea1189Δflg-4Deletion of EAM_2569-2581; genes fliEFGHIJKLMNOPQ of a putative flagellum structureThis study
Ea1189ΔcrlDeletion of EAM_0898; putative curlin regulatorThis study
Ea1189ΔeaeDeletion of EAM_3759; putative invasin structureThis study