Table 2.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristic(s)aReference
Strains
    E. coli DH5αCloning strain
    UA159Streptococcus mutans reference strain2
    UA140Wild-type Streptococcus mutans40
    ATCC 25175Wild-type Streptococcus mutans9
    L13Wild-type Streptococcus mutans48
    CL1Wild-type Streptococcus mutans50
    ZX-4IFDC1UA159 ΔnlmA, Emr, p-Cl-PhesThis study
    ZX-4IFDC2UA159 ΔnlmA, Emr, p-Cl-PhesThis study
    ZX-4IFDUA159 ΔnlmA, Ems, p-Cl-PherThis study
    ZX-4IFD2UA140 ΔnlmA, Ems, p-Cl-PherThis study
    ZX-4IFD325175 ΔnlmA, Ems, p-Cl-PherThis study
    ZX-4IFD4L13 ΔnlmA, Ems, p-Cl-PherThis study
    ZX-4IFD5CL1 ΔnlmA, Ems, p-Cl-PherThis study
    ZX-6IFDUA159 ΔnlmD, Ems, p-Cl-PherThis study
    ZX-5IFDUA159 ΔnlmC, Ems, p-Cl-PherThis study
    ZX-46IFDUA159 ΔnlmA, ΔnlmD, Ems, p-Cl-PherThis study
    ZX-45IFDUA159 ΔnlmA, ΔnlmC, Ems, p-Cl-PherThis study
    ZX-56IFDUA159 ΔnlmC, ΔnlmD, Ems, p-Cl-PherThis study
    ZX456IFDUA159 ΔnlmA, ΔnlmC, ΔnlmD, Ems, p-Cl-PherThis study
Plasmids
    pDL278E. coli-S. mutans shuttle vector
    pWTphespGEM::pheS, AprThis study
    pMTphespGEM::pheS*, AprThis study
    pIFDC1pDL278::ldhp::pheS*::ermAM, Spr, EmrThis study
    pMTmphespGEM::mpheS*, AprThis study
    pIFDC2pDL278::ldhp::mpheS*::ermAM, Spr, EmrThis study
  • a Apr, ampicillin resistance; Spr, spectinomycin resistance; Kmr, kanamycin resistance; Emr, erythromycin resistance; p-Cl-Pher, p-chloro-phenylalanine resistance.