Table 3.

Predicted versus experimental coverage and percent abundance of reads for each phage in the mock metagenome

PhageVirus typeaGenome size (bp)GC content (%)Predicted resultExperimental result
Recruitment to reference sequenceMock assembly
No. of readsCoverageAbundance of reads (%)No. of readsCoverageb (± SD)Abundance of reads (%)No. of contigsLongest contig (bp)N50 contig (bp)
T4C166,0003520,0004728.131,04961.6 (14.6)48.23169,170169,170
CateraM153,7666514,0003619.77,59218.0 (8.6)11.8485,29885,298
FruitloopM58,4716212,0008216.88,74553.3 (18.9)13.6158,30858,308
GumballM64,8076010,0006214.09,99956.4 (18.8)15.5164,80764,807
OmegaM110,865618,0002911.23,36210.9 (5.2)5.2641,62620,153
PorkyM76,312634,000215.62,0389.7 (5.8)3.2734,43417,944
SolonM49,487642,000162.87155.2 (3.4)1.11210,5707,363
P-SS2Cy107,530521,0003.71.47572.5 (2.2)1.2812,9241,313
S-SM1Cy174,079412000.50.32050.4 (0.9)0.350915463
Total71,20064,718 (257 unassigned, 64,462 assigned)172 (6 unassigned, 166 assigned)
  • a C, coliphage; M, mycobacteriophage; Cy, cyanophage.

  • b Coverage is defined as number of bases per position in the consensus sequence.