Table 5.

Protein changes for BTE-expressing strains versus BTE-H204A-expressing strains cultured in EZ rich defined medium supplemented with 0.2% glucose in shake flasks (EZ glucose) or in EZ rich defined medium supplemented with 0.4% glycerol (EZ glycerol)a

LocusProteinFold change between BTE-expressing strains vs BTE-H204A-expressing strains
EZ glucose, BTE+ ACCEZ glycerol
BTE+ ACCBTE+ ACC+
Early stationaryMid-stationaryLate logMid-stationaryLate logMid-stationary
PutA2.102.20−4.32
b1304PspA5.522.28
b1493GadB4.63
b1611FumC3.53
b1743Spy5.24
b2323FabB−4.20−3.46−2.36−5.85−2.46−7.00
b3506Slp2.10−2.11
  • a Cultures were grown in fermentors and sampled at the times specified in the text. P values for all fold changes shown were less than 0.05. No significant changes in expression levels were measured at the mid-log phase sampling point for EZ glucose cultures.