Table 1.

Plasmids and strains used in this study

Plasmid or strainDescription and/or relevant characteristicsaSource or reference
Plasmids
    pMU1647Cloning vector; contains cpbp-tdk and gapDHp-cat-hpt operonThis study
    pMU1657HPT KO vector with cpbp-hpt and gapDH-cat outside deletion flankThis study
    pMU1758Lactate dehydrogenase deletion vectorThis study
    pMU1777Lactate dehydrogenase deletion vector without yeast machineryThis study
    pMU1817Phosphotransacetylase deletion vectorThis study
Strains
    C. thermocellum
        M0003Wild-type DSM 1313DSMZb
        M1354DSM 1313 ΔhptThis study
        M1375M1354 ΔldhThis study
        M1448M1354 ΔptaThis study
        M1434M1375 ΔptaThis study
        M1570M1434 evolvedThis study
    T. saccharolyticum
        ALK2Δldh Δpta-ack24
  • a HPT, hypoxanthine phosphoribosyl transferase; KO, knockout.

  • b Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany.