TABLE 1.

S. cerevisiae strains used in this study

StrainGenotype
CEN.PK 113-7DMATa, reference strain
IMK157 ura3ΔMATa pyc1::kanMx pyc2::ILV2smr ura3-52
IMK299MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52
IMW012MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (cured); derived from IMY017
IMY007 empty vectorMATa pyc1::kanMx pyc2::ILV2smr ura3-52 (pUDC1)
IMY016MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (MB5573)
IMY017MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (MB5573*); evolved
IMY019MATa pyc1::kanMx pyc2::ILV2smr ura3-52 (MB5573)
IMY024MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (MB5573*)
IMY025MATa pyc1::kanMx pyc2::ILV2smr ura3-52 (pUDC10)
IMY026MATa pyc1::kanMx pyc2::ILV2smr ura3-52 (pUDC9)
IMY027MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (pUDC10)
IMY028MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (pUDC9)
IMY029MATa pyc1::kanMx pyc2::ILV2smr pdc2::loxP::hph::loxP ura3-52 (pUDC9); derived from IMY017
BY4741 mae1ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mae1::kanMX
IMY030MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mae1::kanMX (pUDC1)
IMY031MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mae1::kanMX (MB5573)
IMY032MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mae1::kanMX (MB5573*)