TABLE 2.

Summary of ORFs and gene products of TPA2

ORFaNo. of amino acids (% identity)bPredicted function (conserved motif)cMatchdE valuee
1146Unknown
2193Unknown
3146Unknown
4172Unknown
579Unknown
653Unknown
7139Unknown
8140Unknown
terL554 (51)Large terminase (Pfamn03237)Gp7, Mycobacterium phage Rosebush1e−145
10240Unknown
1179Unknown
12118Unknown
1395Unknown
14113Unknown (Pfam05305)
ruvC192 (40)RuvC resolvase (cd00529)Mycobacterium phage Pipefish6e−28
16114 (42)Unknown (Pfam07098)Gp6, Mycobacterium phage Nigel1e−19
17611 (52)Putative structural proteinGp12, Mycobacterium phage Qyrzula4e−164
18793 (52)Structural protein (Pfam04233)Gp12, Mycobacterium phage Pipefish0.0
1964 (53)Putative structural proteinGp13, Mycobacterium phage Pipefish8e−12
2072 (0)Unknown
21414 (69)UnknownGp2, Mycobacterium phage Cooper6e−167
22260 (45)UnknownGp13, Mycobacterium phage Orion5e−53
23199 (34)UnknownGp16, Mycobacterium phage Pipefish8e−17
24101 (59)UnknownGp15, Mycobacterium phage Cooper1e−21
25151 (49)UnknownGTE5p031, Gordonia terrae phage GTE55e−34
26147Unknown
27268 (67)Major tail proteinGp21, Mycobacterium phage Pipefish2e−102
28256 (50)Putative tail assemblyGp23, Mycobacterium phage Pipefish1e−64
29234 (48)Putative tail assemblyGp23, Mycobacterium phage Qyrzula3e−45
3097 (68)UnknownGp20, Mycobacterium phage Nigel2e−06
31147 (51)Tail component (Pfam04883)Gp25, Rhodococcus phage ReqiPine55e−29
32145 (49)UnknownGp27, Mycobacterium phage Pipefish1e−29
332,386 (36)Tape measure protein (Pfam10145)TMP, Mycobacterium abscessus2e−102
34331 (49)UnknownGp30, Mycobacterium phage pipefish3e−96
35292 (49)UnknownGp30, Mycobacterium phage Colbert3e−69
36227Unknown
37171Unknown
38188Unknown
39209 (59)UnknownGp36, Mycobacterium phage Rosebush3e−59
40196 (65)UnknownGp32, Mycobacterium phage Nigel3e−70
41184 (45)UnknownGp38, Mycobacterium phage Phlyer9e−39
42133 (37)UnknownGp39, Mycobacterium phage Rosebush2e−13
4358 (44)UnknownGp37, Mycobacterium phage Cooper6e−04
44137 (46)Putative tail fiberGp43, Mycobacterium phage Pipefish3e−23
4588 (50)UnknownGp44, Mycobacterium phage Pipefish3e−12
46364 (36)Putative tail fiberGp6, Mycobacterium phage Jasper4e−15
47218Unknown
4894 (65)Putative regulatorGp45, Mycobacterium phage Pipefish2e−18
4950 (56)UnknownGp46, Mycobacterium phage Pipefish6e−04
5083Unknown
51113Unknown
52131Unknown
53244 (32)UnknownGp41, Mycobacterium phage PBI13e−18
54294 (27)Putative holingRhodococcus opacus1e−04
lysA536 (59)Lysin (Pfam01510, Pfam08310)Nocardia facinica4e−103
56169 (46)UnknownGp48, Mycobacterium phage Phlyer8e−26
57435 (39)UnknownGp47, Mycobacterium phage Cooper1e−82
58549 (56)Helicase (Pfam04851) (Pfam00271)Gp50, Mycobacterium phage Rosebush4e−167
59161 (40)UnknownGp51, Mycobacterium phage Cooper4e−15
6086Unknown
61870 (51)Primase (Pfam09250)Gp52, Mycobacterium phage Phaedrus0.0
62178 (47)UnknownHypothetical, Jonesia denitrificans7e−19
63626 (52)DNA polymerase I (Pfam01612, Pfam00476)Gp54, Mycobacterium phage Cooper6e−174
64259Unknown
6585Unknown
66202Unknown
67136Unknown
68150Unknown
69124Unknown
7099Unknown
7185Unknown
72208 (27)UnknownGp65, Mycobacterium phage Rosebush2e−07
7379Unknown
74151Unknown
75112 (63)Putative endonucleaseGp85, Mycobacterium phage Scoot17C7e−22
76182 (46)UnknownGp72, Mycobacterium phage Phaedrus1e−29
77102Unknown
78110 (50)Metallophoesterase (COG4186)Gp66, Rhodococcus jostii1e−17
  • a ORFs were numbered consecutively, with the exceptions of terL, ruvC, and lysA.

  • b Percent identity is based on the best match when a BLAST P analysis was performed.

  • c Predicted function is based on amino acid identity, conserved motifs, N-terminal sequencing, and gene location within functional modules.

  • d The most closely related gene (only if named) and the name of the organism.

  • e The probability of obtaining a match by chance as determined by BLAST analysis. Only values of less than 10−4 were considered significant.