TABLE 1.

Results of the growth assays carried out with L. casei BL23 and selected TCS-defective mutants under different growth conditionsa

ConditionGrowth parameterResultb
BL23TC01TC06TC12
Bile, 0.5%μmax0.17 ± <10−2NGNG0.08 ± <10−2
Change in OD1.15 ± 0.14NGNG0.52 ± 0.03
0.6 M NaClμmax0.23 ± 0.01NGNG0.21 ± 0.01
Change in OD1.83 ± 0.04NGNG1.81 ± 0.03
pH 3.75μmax0.07 ± <10−20.03 ± <10−20.03 ± 0.01NG
Change in OD0.56 ± 0.070.19 ± 0.060.14 ± 0.01NG
42°Cμmax0.26 ± 0.010.24 ± <10−20.17 ± 0.010.22 ± 0.02
Change in OD2.19 ± 0.102.07 ± 0.150.46 ± 0.031.02 ± 0.05
  • a See Tables S4A and B in the supplemental material for data of all mutants and the results of the ANOVA.

  • b The data shown are means and standard deviations. NG, no growth.