Parameter | HHP ([biphasic] linear model) | Heat ([biphasic] linear with shoulder model) | ||||
---|---|---|---|---|---|---|

Wild type | ctsR variant | Immotile variant | Wild type | ctsR variant | Immotile variant | |

N_{0,measured} (log CFU/ml) | 9.4 | 9.7 | ||||

N_{0,extrapolated} (log CFU/ml) | 10.3 | 10.0 | 9.6 | 9.7 | 9.6 | 9.6 |

D_{1} (min) | 1.5 | 7.7* | 16.3** | 0.86 | 3.1** | 4.5** |

D_{2} (min) | 7.1 | 7.6 | ||||

f_{res} | 2.1 × 10^{−5} | 6.5 × 10^{−6} | 1.0 × 10^{−7} | 1.6 × 10^{−4} | 6.5 × 10^{−6} | 1.0 × 10^{−7} |

Sl (min) | 3.6 | 7.8*** | 5.2 |

↵a Cells were treated under conditions of 350 MPa, 20°C (HHP) or at 55°C (Heat) in ACES buffer.

*N*_{0,extrapolated}is the model-based-fit amount of cells at*t*_{0}.*N*_{0,measured}is the measured amount of cells at*t*_{0}.*D*is the decimal reduction time, where*D*_{1}and*D*_{2}are the*D-*values of the first and second part, respectively, of the biphasic linear inactivation curve.*f*_{res}is the fraction of resistant cells in the population based on*N*_{0,measured}for the wild type, where the*f*_{res}given for the wild type is the total fraction of resistant cells (both stably and temporarily resistant) and the*f*_{res}given for each of the two variants is the fraction of that specific variant in the wild-type population, assuming no shoulder for the two variants (see behavior in Fig. 1b) and taking into account the shoulder in the wild type by using*N*_{0,measured}.*Sl*is the shoulder length. *, statistical analysis (*P*< 0.05) showed that the*ctsR*variant's*D*-value for HHP was only higher than the*D*-value of the sensitive fraction of the population of the wild type; **, the*D*-value for HHP of the immotile variant and both variants'*D*-values for heat were significantly different from the*D*-values of the sensitive and resistant fractions of the wild-type population; ***, the shoulder length of the*ctsR*variant was higher than that of the wild type for heat inactivation.