Table 1.

Biochemical purification of bacteriocin L-1077 using specified procedures

Sample purification stepProtein (mg/ml)Sp act (AU/mg protein) against C. jejuniPurity (%)
Culture supernatant1.34350
Gel filtration on Superose 12 HR 10/300.0280,00013.3
Binding to SP-Sepharose FF0.015107,00076.4
Hydrophobic-interaction chromatography on Phenyl Sepharose 6 Fast Flow0.007229,00095.7