Table 2.

Names and descriptions of one-step USER vectors for inserting YFG into IS1

VectorMarkerPromoterTerminatorUSER cassette/cloning cassetteIntegration site
pU1111-1argBPgpdATtrpCAsiSI/Nb.BtsIIS1
pU1211-1argBPalcATtrpCAsiSI/Nb.BtsIIS1
pU2111-1pyrGPgpdATtrpCAsiSI/Nb.BtsIIS1
pU2211-1pyrGPalcATtrpCAsiSI/Nb.BtsIIS1
pU3111-1bleaPgpdATtrpCAsiSI/Nb.BtsIIS1
pU3211-1bleaPalcATtrpCAsiSI/Nb.BtsIIS1
  • a ble confers resistance to the genotoxin bleomycin.