Table 1

Oligonucleotides used to amplify specific regions from the relevant strain's chromosome for the purpose of Sanger sequencing

OligonucleotideSequence (5′→3′)
rpoD_fwdATGGAGCAAAACCCGCAGTC
rpoD_revTTAATCGTCCAGGAAGCTAC
ylbE_fwdATGTTTACATCAGTGGCGCA
ylbE_revTCACTTCCCCTGCTCCAGTA
kdpD_fwdATGAATAACGAACCCTTACG
kdpD_revTCACATATCCTCATGAAATT
ybhN_fwdATGAGTAAATCACACCCGCG
ybhN_revTCACATCGCCGCTTCATTTT
rpsA_fwdATGACTGAATCTTTTGCTCA
rpsA_revTTACTCGCCTTTAGCTGCTT
pncB_fwdATGACACAATTCGCTTCTCC
pncB_revTTAACTGGCTTTTTTAATAT
fabA_fwdATGGTAGATAAACGCGAATC
fabA_revTCAGAAGGCAGACGTATCCT
yddB_fwdATGAAGCGAGTTCTTATTCC
yddB_revTTAAAATTTCATGCTGACAT
dgsA_fwdGTGGTTGCTGAAAACCAGCC
dgsA_revTTAACCCTGCAACAGACGAA
pykF_fwdATGAAAAAGACCAAAATTGT
pykF_revTTACAGGACGTGAACAGATG
yejM_fwdATGGTAACTCATCGTCAGCG
yejM_revTCAGTTAGCGATAAAACGCT
tktB_fwdATGTCCCGAAAAGACCTTGC
tktB_revTCAGGCACCTTTCACTCCCA
mreD_fwdGTGGCGAGCTATCGTAGCCA
mreD_revTTATTGCACTGCAAACTGCT
rpsJ_fwdATGCAGAACCAAAGAATCCG
rpsJ_revTTAACCCAGGCTGATCTGCA
perR_fwdATGAAGCTCTTAGCAAAAGC
perR_revTCAACGAATTTTACCCAGAT
malQ_fwdATGGAAAGCAAACGTCTGGA
malQ_revCTACTTCTTCTTCGCTGCAG
malT_fwdATGCTGATTCCGTCAAAACT
malT_revTTACACGCCGTACCCCATCA
yhhZ_fwdATGAGTAATATTGTTTACCT
yhhZ_revTCATTTTGTGTGGTCCATAA
malS_fwdATGAAACTCGCCGCCTGTTT
malS_revTTACTGTTGCCCTGCCCAGA
spoT_fwdTTGTATCTGTTTGAAAGCCT
spoT_revTTAATTTCGGTTTCGGGTGA
wzzE_fwdATGACACAACCAATGCCTGG
wzzE_revCTATTTCGAGCAACGGCGGG
rffT_fwdATGACTGTACTGATTCACGT
rffT_revTCATGCGACCTCCCTGGCGG
glpF_fwdATGAGTTAAACATCAACCTT
glpF_revTTACAGCGAAGCTTTTTGTT
treB_fwdATGATGAGCAAAATAAACCA
treB_revTTAAACAATGTCCAGCGTGC
idi_fwdATGCAAACGGAACACGTCAT
idi_revTTATTTAAGCTGGGTAAATG
yidE_upstream_fwdCCAATACCTAATCCTATGCC
yidE_upstream_revTCGTAAACGGTTTACTGCAT
ppiC_upstream_fwdAGCTTGCCGAAATCGGCCCC
ppiC upstream_revCTTACAGAGGGTATCTTAAT
yegTfbaB_upstream_fwdTCATGTCCGGGGAGATAAAG
yegTfbaB_upstream_revAAACCGCTTTTACTTAACCA
rydC_upstream_fwdCGCATGATGCCGCGTAAACG
rydC_upstream_revTGTGAGATCCCCCCTTTCGA
yajD_upstream_fwdTGGCATCTGCGTTGGCTCTG
yajD_upstream_revAACTCGCGGGAACAGCGACC
gltP_upstream_fwdTATGGCAAAAAGTGATGGAT
gltP_upstream_revTCGCGGCTGTCGCTATGGTA
yqjF_upstream_fwdATCCTAATATGCTGGTCCGC
yqjF_upstream_revGTACCCGCGTAGCCAGTAAT