Table 3

Identification of SNPs and their evolutionary history

Tmax (°C)aMutation in strainbGenePosition of SNP in gene (gene length)Genome positionMutationAa changec
MG1655EVG1031EVG1041EVG1058EVG1064
XXXXXylbE114 (1,258)547694A→G
46.9XXXXkdpD1448 (2,685)722190A→GVal 483 Ala
XXXXdgsA607 (1,221)1665982C→TGlu 203 Lys
XXXXrpoD293 (1,842)3211361T→AVal 98 Glu
XXXXrpsJ95 (312)3451198G→CThr 32 Ser
XXXXyhhZ1057 (1,179)3580942A→GThr 353 Ala
XXXXspoT1091 (2,109)3821513A→GGlu 364 Gly
XXXXUpstream of yidE3864388A→G
XXXXtreB338 (1,422)4463866A→TVal 113 Glu
XXXXperR778 (894)268629C→TGlu 260 Lys
49.3XXXmalQ1769 (2,085)3546324A→GLeu 590 Pro
XXXwzzE595 (1,047)3967648G→CAla 199 Pro
XXXrpsA812 (1,674)692029C→TAla 271 Val
XXXpykF342 (1,413)1754063T→G
49.6XproP850 (1,503)4229239A→CSer 284 Arg
XXybhN67 (957)821655C→TVal 23 Met
XXyddB2047 (2,373)1573597G→THis 683 Asn
XXpncB52 (1,203)989528A→GTyr 18 His
XXmreD335 (489)3396563A→GLeu 112 Pro
49.7XmalT1751 (2,706)3552857C→GAla 584 Gly
XmalS1134 (2,031)3736653A→G
XUpstream of ppiC3957957C→T
XrffT180 (1,080)3975727G→A
XglpF7 (846)4116107G→AGln 3 Stp
XUpstream of gltP4292389A→T
XUpstream of yajD429789A→G
XfabA108 (519)1015586C→TMet 36 Ile
XUpstream of rydC1489550T→C
XUpstream of yegT and fbaB2176752T→C
XyejM1069 (1,761)2283466G→AAla 357 Thr
XtktB1499 (2,004)2579156T→GVal 500 Gly
Xidi395 (549)3031481A→TAsn 132 Ile
XUpstream of yqjF3248421C→T
  • a Tmax indicates the temperature of the Evolugator incubation chamber when the strain was removed. —, original strain (not isolated from the machine).

  • b Shaded X, the strain in which the mutation was first observed; X, strains that retain the mutation.

  • c Stp, stop codon.