Table 4

Growth of wild-type and thermotolerant mutant strains on M9 minimal plates

StrainGrowtha
30°C, without37°C43°C46°C48.5°C
WithoutWithWithoutWithWithoutWithWithoutWith
DMDGMDGDGMDGDGMDGDGMDG
MG1655++++++++++++
EVG1031+++++++++
EVG1041+++++++++++++
EVG1058+++++++++++++
EVG1064+++
  • a D, M, and G define the carbon sources used in the minimal medium. D, dextrose; G, glycerol; M, maltose. + and – refer to growth or no growth on plates. M9 medium is either unsupplemented (without) or supplemented (with) with the amino acids and cofactors defined in Materials and Methods.