Table 5

Analysis of fatty acids from EVG1058 and EVG1064a

Fatty acid% total fatty acids ± SD
37°C48°C
EVG1058EVG1064EVG1058EVG1064EVG1064 fabA strainEVG1064 folE strain
Saturated
    C12:04.6 ± 0.34.4 ± 0.26.3 ± 0.6**‡5.0 ± 0.4**‡4.3 ± 0.0*§3.8 ± 0.1*§
    C14:09.4 ± 1.69.8 ± 1.411.4 ± 1.012.2 ± 0.18.3 ± 0.4**§9.2 ± 0.2**§
    C16:030.1 ± 1.330.8 ± 2.436.3 ± 2.8**‡41.6 ± 1.3**‡39.8 ± 2.4**§44.6 ± 1.1**§
    C17:01.0 ± 0.91.0 ± 1.00.6 ± 0.30.4 ± 0.1NDND
    C18:00.2 ± 0.10.3 ± 0.10.8 ± 0.20.9 ± 0.11.2 ± 0.11.5 ± 0.2
Unsaturated
    C16:1 Δ913.8 ± 2.611.3 ± 5.216.1 ± 0.8**‡13.4 ± 1.1**‡8.6 ± 1.26.2 ± 1.2
    C16:1 Δ110.21 ± 0.03**†0.15 ± 0.02**†NDND0.5 ± 0.0ND
    C18:1 Δ1111.5 ± 0.89.1 ± 1.86.7 ± 2.4**‡2.8 ± 0.3**‡6.9 ± 1.5**§2.1 ± 0.5**§
Other
    3-OH-C14:09.7 ± 0.49.3 ± 0.913.4 ± 2.511.3 ± 1.510.6 ± 0.39.8 ± 0.6
    C17:0 cyc15.6 ± 2.618.4 ± 4.86.8 ± 2.311.1 ± 1.214.4 ± 0.3*§17.2 ± 0.5*§
    C17:1 Iso INDNDNDND2.7 ± 0.83.4 ± 1.1
    C19:0 cyc ω8c1.2 ± 0.51.5 ± 1.00.1 ± 0.20.2 ± 0.21.3 ± 0.21.0 ± 0.1
Saturated/unsaturated1.8 ± 0.32.4 ± 0.82.5 ± 0.3**‡3.7 ± 0.3**‡3.4 ± 0.7**§7.4 ± 1.6**§
Saturated/unsaturated + cyc1.1 ± 0.01.2 ± 0.11.9 ± 0.2**‡2.2 ± 0.02**‡2.2 ± 0.2**§2.6 ± 0.2**§
  • a The same symbols in a row (†, ‡, §) indicate paired values that are significantly different when compared using t tests. Asterisks indicate P values: *, P < 0.005; **, P ≤ 0.05. Error bars indicate ±1 standard deviation. cyc, cyclopropane fatty acid; Δ, position of the double bond or cyclopropane ring relative to the carboxyl group; 3OH, β-hydroxyl group; Iso I, a terminally branched-chain fatty acid; ND, not detected.