Table 1

Primer sequences

Primer nameaPrimer sequence (5′→3′)
Hydrox, Amido, Mel1,2 ForACGGCCAGTGCCAAGCTTCCTAGAGGTCCTTGTCTGACGGAGC
Hydrox, Amido, Mel1,2 RevTGACATGATTACGAATTCGTTCCTCACTGGATGAAAGGCAGTC
MelC1,2 ForACGGCCAGTGCCAAGCTTCGACTTCGACACGCTCGTCCGTACG
MelC1,2 RevTGACATGATTACGAATTCCGTGAGCGACACCACGGACATGTCC
Phospholipase ForACGGCCAGTGCCAAGCTTGCATGGAGACCGTCGACAAGAAGG
Phospholipase RevTGACATGATTACGAATTCCCTCACCCTGTCCGATTGTCC
WhiG ForACGGCCAGTGCCAAGCTTGCACTGCTGCTCGGACAGC
WhiG RevTGACATGATTACGAATTCCCAGCGTGAGCGCTGGTCAGC
S15 ForACGGCCAGTGCCAAGCTTGCTTTGGCGAGACTCTCGATCTCG
S15 RevTGACATGATTACGAATTCCAGAGTTCTCAGGTTCGGCGATTACG
M28 ForACGGCCAGTGCCAAGCTTGGAAGATTCGTTGCTTGCTGC
M28 RevTGACATGATTACGAATTCGGTAGCGGTCCTGGAAGATCAGC
Phospholipase del ForTCCCATGTCTACCGGCGGGTAACTGCCGCTGCTTTGATGGTGTAGGCTGGAGCTGCTTC
Phospholipase del RevTTACCGCCCCGACGCGATCACCGGGTAGTGGTCGGAGAGCATATGAATATCCTCCTTA
WhiG del ForACACCCACGTTTCAGTTGAGAGGCTCAAGGTCACGTATGGTGTAGGCTGGAGCTGCTTC
WhiG del RevTCAGTCCTGGTCGGTGAGCTTGGCCCGCAGCTGAAGGACCATATGAATATCCTCCTTA
S15 del ForCGCCGGCATCATGCCGCCGACATCCGGGCAGATGTTATGGTGTAGGCTGGAGCTGCTTC
S15 del RevTCAGCGCATCACTGCAATCTCGAGGCGGGAGGGATGCGCCATATGAATATCCTCCTTA
M28 del ForCCGTACGGTCGAGTGCCGAGTGCGAGGCGGAGCGGGATGGTGTAGGCTGGAGCTGCTTC
M28 del RevTCAGGCGGGCGAGGTGAGAGCGCGGCGGGCTTGCGCCAACATATGAATATCCTCCTTA
MelC2 del ForTTCAACTGACCTTTCCAGCACCGGAGTTCATCTATCATGTGTGTAGGCTGGAGCTGCTTC
MelC2 del RevGAACGTGTAGTGGGCCGTGTGGTCCAGCAGATCCGCCGGCATATGAATATCCTCCTTA
Hydrox, Amido, Mel1,2 del ForCCTTGAGTAACTCTTGAGCGATTGTGGGATGTGCGCGTGTGTGTAGGCTGGAGCTGCTTC
Hydrox, Amido, Mel1,2 del RevCATACGAAACACCAACTGGCCCTTTTCCGGCGGCATATGCATATGAATATCCTCCTTA
Amido del ForGCCCATGGGCCGGGAGATGCGCGAGCGTGCTGCGTCATGTGTGTAGGCTGGAGCTGCTTC
Amido del RevTCAGTGGCCCGTGTCGCAGCAGCCCTCCCGCCGCATCAGCATATGAATATCCTCCTTA
Hydrox del ForCCTTGAGTAACTCTTGAGCGATTGTGGGATGTGCGCGTGTGTGTAGGCTGGAGCTGCTTC
Hydrox del RevTCAGCTCTTCGTGCCGTGCAGGGTCCGTGCCACCTTCGGCATATGAATATCCTCCTTA
  • a Hydrox, hydroxylase; Amido, amidohydrolase; del, deletion.