Table 5

Characteristics of ORFs in the C. sakazakii O6 O-antigen gene clustera

ORFGeneGene position (length [aa])G+C content (%)Pfam conserved domain(s) (Pfam no./e-value)Similar protein(s), strain(s) (GenBank accession no.)% Identity (aa overlap)Putative function of protein
6-1wepL47–829 (260)31.30Glycosyltransferase family 2 (PF00535/4.2e-25)Glycosyltransferase, F. bacterium BAL38 (ZP_01733772.1)38 (118)Glycosyltransferase
6-2wepM813–2072 (413)30.13Capsule polysaccharide biosynthesis protein (PF05159/1.7e-22)Capsule polysaccharide biosynthesis protein family protein, Marinomonas sp. (ZP_010075937.1)34 (334)Capsule polysaccharide biosynthesis protein family protein
6-3wzx2065–3270 (401)32.18Polysaccharide biosynthesis protein (PF01943/2.6e-26)Polysaccharide biosynthesis protein, B. helcogenes P36-108 (YP_004162007)27 (344)Flippase
6-4wzy3260–4486 (408)30.65Wzy, P. mirabilis (ADL32282)26 (195)Polymerase
6-5wepN4488–5327 (279)32.75Core-2/I-branching enzyme (PF02485/2.1e-17)Glycosyltransferase family 14, M. paludis DSM (ZP_07747376)26 (294)Glycosyltransferase
6-6wepO5324–6160 (278)34.52Glycosyltransferase family 2 (PF00535/2e-23)Glycosyltransferase WceM, Erwinia sp. strain Ejp617 (ADP12985.1)50 (267)Glycosyltransferase
6-7wepP6185–7225 (346)32.76Acyltransferase family (PF01757/1.2e-23)Exopolysaccharide biosynthesis protein, Erwinia sp. strain Ejp617 (ADP10993)32 (352)Acetyltransferase
  • a aa, amino acid(s).