Table 6

Characteristics of ORFs in the C. sakazakii O7 O-antigen gene clustera

ORFGeneGene position (length [aa])G+C content (%)Pfam conserved domain(s) (Pfam no./e-value)Similar protein(s), strain(s) (GenBank accession no.)% Identical (aa overlap)Putative function of protein
7-1wzx69–1358 (430)33.02Polysaccharide biosynthesis protein (PF01943/8.6e-23)Polysaccharide biosynthesis protein Wzx, V. vulnificus (ABD38622)31 (404)Flippase
7-2wepQ1286–2741 (485)29.42Glycosyltransferase group 1 (PF00534/5.1e-12)Glycosyltransferase group 1, A. baumannii ABNIH2 (EGT89242.1)35 (264)Glycosyltransferase
7-3wepR2743–3918 (391)31.71Glycosyltransferase group 1 (PF00534/4.3e-13)Glycosyltransferase group 1, Marinomonas sp. (YP_001339674.1)28 (392)Glycosyltransferase
7-4fnlA3911–4945 (344)41.06Polysacc_synt_2 (PF02719.8/2.7e-109)l-Fucosamine synthetase, E. coli (ABM53659)93 (344)Fucosamine synthetase
7-5fnlB4947–6050 (367)41.30NAD-dependent epimerase/dehydratase family (PF01370.14/1.2e-13)FnlB, epimerase, S. dysenteriae (ACA24843)78 (367)Epimerase
7-6fnlC6050–7180 (376)40.93UDP-N-acetylglucosamine 2-epimerase (PF02350.12/5.5e-95)C-2 epimerase, E. coli MS 145-7 (ZP_07692194.1)92 (376)Epimerase
7-7wbuB7180–8388 (402)42.10Glycos_transf_1 (PF00534.13/1.6e-09)l-Fucosamine transferase, S. dysenteriae (ACA24845)76 (398)Glycosyltransferase
7-8wbuC8379–8783 (135)42.71Domain of unknown function (DUF1971/0.0009)WbuC, S. dysenteriae (ACA24846)69 (131)Glycosyltransferase
  • a aa, amino acid(s).