Table 2

Simplex qPCR calibration curves and performance metrics

AssayEquationEaR2LLOQb
CperfY = 38.8 − 3.55X0.950.97835.36
uidAY = 41.4 − 3.69X0.860.99237.69
Entero1Y = 37.5 − 3.15X1.080.96533.99
GenBac3Y = 37.7 − 3.44X0.950.99334.17
  • a Amplification efficiency [E = 10(−1/slope) − 1].

  • b Lower limit of quantification (mean Cq of 10-copy-standard data).