Table 3

Multiplex qPCR calibration curves, performance metrics, and interference parameters

AssayEquationEaR2LLOQbIAC ROQcInterference CqCompetition Cq
CperfY = 38.3 − 3.44X0.950.97835.361 to 236.931.4
uidAY = 39.8 − 3.30X1.010.96536.38136.532.47
Entero1Y = 37.7 − 3.34X0.990.97534.091 to 237.731.0
GenBac3Y = 37.5 − 3.42X0.960.99533.961 to 3.635.525.19
  • a Amplification efficiency [E = 10(−1/slope) − 1].

  • b Lower limit of quantification (mean Cq of 10-copy-standard data).

  • c Internal amplification control range of quantification.