Table 1

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    S. coelicolor
        M145Parental strain, SCP1 SCP219
        TR2951M145, Sco2951::Tn5062 (Amr)This study
        TR5261M145, Sco5261::Tn5062 (Amr)This study
        TRDM145, Sco2951::Tn5062 (Amr), Sco5261::Tn 5066 (Hygr)This study
        TR2951cTR2951 derivative carrying the integrative plasmid pTR2951 (Kmr)This study
        TR5261cTR5261 derivative carrying the integrative plasmid pTR5261 (Kmr)This study
        TRDc2951TRD derivative carrying the integrative plasmid pTR2951 (Kmr)This study
        TRDc5261TRD derivative carrying the integrative plasmid pTR5261 (Kmr)This study
    E. coli
        DH5αE coli K12 F lacU169(f80lacZDM15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA112
        BL21λ(DE3)E. coli B F ompt rB mB (DE3)33
        ET 12567supE44 hsdS20 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-1 dam dcm hsdM (Cmr)29
Plasmids
    pET28aPhagemid vector for expression of recombinant proteins under the control of strong T7 transcription and translation signals (Kmr)Novagen
    PCR-BluntUsed for cloning PCR products (Apr)Invitrogen
    pTR285pRT802 derivative carrying the PermE*promoter2
    pTR252pET28a with an insert carrying the sco2951 His tag fusion gene under the control of strong T7 transcription and translation signalsThis study
    pTR5261pRT285 derivative plasmid carrying the sco5261-His tag gene under the control of PermE*This study
    pTR2951pRT285 derivative plasmid carrying the sco2951-His tag gene under the control of PermE*This study