Table 2

Kinetic parameters of SCO2951 (NAD+-ME) and SCO5261 (NADP+-ME)a

ProteinReactionSubstrateVmax (U mg−1)Km (mM)
SCO5261DirectNADP36.97 ± 1.630.107 ± 0.015
l-Malate6.4 ± 0.6
ReversePyruvate0.0824 ± 0.011418.1 ± 3.7
SCO2951DirectNAD2.35 ± 0.214.3 ± 0.9
l-Malate8.1 ± 1.4
  • a Recombinant proteins were expressed and purified using affinity columns. NAD+- and NADP+-ME activities were determined spectrophotometrically at 30°C by monitoring NADH and NADPH production at 340 nm, respectively. Results are the means from three determinations ± standard errors.