Table 1

Strains and plasmids used in this study

Strain or plasmidGenotype and/or phenotypeaSource or reference
Strains
    C. burnetii
        Nine Mile phase II (NMII)Phase II, clone 4Beare et al. (6)
        NMII/Tn7-CAT-311P-MC-sacBNMII containing a Tn7 transposon inserted between CBU1787 and CBU1788; CmrThis study
        ΝΜΙΙ/ΔdotA-loxPCre-lox-mediated dotA deletion mutant; KanrThis study
        ΝΜΙΙ/ΔdotAdotA deletion mutant; KanrThis study
        ΝΜΙΙ/ΔdotBdotB deletion mutant; KanrThis study
    E. coli
        TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
Plasmids
    pJC84ColE1 ori; KanrWehrly et al. (48)
    pUC19pUC ori; AmprInvitrogen
    pJB-KanpJB2581 containing kan driven by 1169P; KanrOmsland et al. (27)
    pJB-CATpJB2581 containing cat driven by 1169P; CmrOmsland et al. (27)
    pJC-CATpJC84 containing cat driven by 1169P; CmrThis study
    pJC-CAT:DotB5′3′pJC-CAT containing ∼2 kb of dotB 5′ and 3′ flanking sequences; CmrThis study
    pJC-CAT::DotB5′3′-KanpJC-CAT::DotB5′3′ containing a 1169P-Kan cassette cloned into a unique AgeI restriction site; Cmr KanrThis study
    pJC-CAT::DotA5′3′pJC-CAT containing ∼2 kb of dotA 5′ and 3′ flanking sequences; CmrThis study
    pJC-CAT::DotA5′3′-KanpJC-CAT::DotA5′3′ containing a 1169P-Kan cassette cloned into a unique AgeI restriction site; Cmr KanrThis study
    pMiniTn7T-CAT1169P-CAT cloned into pUC18R6K-mini-Tn7T; CmrBeare et al. (3)
    p1898-TnHimar1 transposon vector; CmrBeare et al. (4)
    pJB2581cyaA fusion vector; Ampr CmrBardill et al. (2)
    pJB2581:: 311P-MC311P-MC-CAT-1169P cassette cloned into pJB2581; Ampr CmrThis study
    pMiniTn7T-CAT::311P-MCloxP-311P-MC cassette cloned into pMiniTn7T-CAT; CmrThis study
    pMiniTn7T-CAT::311P-MC-sacBsacB-loxP fragment cloned into pMiniTn7T-CAT::311P-MC; CmrThis study
    pMiniTn7T-Kan-loxP-SacB1169P-Kan-loxP-sacB cassette cloned into EcoRI/SalI-digested pMiniTn7T-CAT; Cmr KanrThis study
    pUC19-Kan-loxP-sacBpUC19 containing a 1169P-Kan-loxP-sacB cassette; KanrThis study
    pUC19-Kan-loxP-sacB::DotA5′flankpUC19-Kan-loxP-sacB containing ∼2 kb of dotA 5′ flanking DNA; KanrThis study
    pJC-CAT-loxPpJC-CAT containing a loxP site; CmrThis study
    pJC-CAT-loxP::DotA3′flankpJC-CAT-loxP containing ∼2 kb of dotA 3′ flanking DNA; KanrThis study
    pBBR1MCS-2::TetR-pTetApBBR1 ori; KanrStarr et al. (39)
    pBSV25-flgBp-creCre expression vector; KanrBestor et al. (8)
    pUC19::1169P-crepUC19 containing cre driven by 1169P; AmprThis study
    pUC19::tetRAP-crepUC19 containing cre driven by tetAP; AmprThis study
    pTnS2::1169P-tnsABCDpTnS2 containing tnsABCD driven by 1169P; AmprBeare et al. (3)
    pMiniTn7T-CAT::icmVP-icmV-dotAicmVP-icmV-dotA cloned into pMiniTn7T-CAT; CmrThis study
    pMiniTn7T-CAT::dotDP-dotDCBdotDP-dotDCB cloned into pMiniTn7T-CAT; CmrThis study
    pJB-CAT-CyaAC. burnetii cyaA fusion vector; CmrVoth et al. (45)
    pJB-CAT-CyaA-A15cbua0015 in pJB-CAT-CyaA; CmrVoth et al. (45)
    pJB-CAT-CyaA-A16cbua0016 in pJB-CAT-CyaA; CmrVoth et al. (45)
  • a Cmr, chloramphenicol resistance; Ampr, ampicillin resistance; Strr, streptomycin resistance; Kanr, kanamycin resistance.