Table 1

Purification of GH78 protein from X. polymorpha (measured by demethylation of methyl ferulate) produced during solid-state fermentation of wheat strawa

Purification stepTotal activity (U)Total protein (mg)Sp act (U/mg)Yield (%)Purification (fold)
Wheat straw extraction57.4910.80.061001.0
Ultrafiltration54.5620.50.0994.91.5
DEAE-Sepharose separation43.968.20.6476.510.7
Superdex Sepharose SEC20.611.51.7935.929.8
Mono Q column separation4.91.92.588.543.0
Biosep-SEC-S-2000 column separation3.81.72.246.637.3
  • a Cultures were harvested after 25 days and extracted with water.