Table 2

Frequency of detection of Sps colonies during the culture of first-crossover integrantsa

Addition of pTBR101-CM to reaction mixture for electroporationNo. of tested colonies (A)No. of Sps second-crossover recombinants (B)Detection frequency (%)
105-A βgal:: pBS423-ΔrepAβgal
    −2,39630.125
    +3,5683,568100
105-A aga:: pBS423-ΔrepAΔaga
    −96310.104
    +1,6451,64399.9
  • a The reaction mixtures were supplemented with pTBR101-CM for electroporation (+) or left unsupplemented (−). Detection frequency was determined by the following equation: (number of Sps second-crossover recombinants/number of tested colonies) × 100.