Table 3

Spacers showing silent or conservative mutations compared to Ma-LMM01 putative proto-spacers

Spacer/phage geneaNucleotide sequencecPredicted amino acid sequencec
298spc124bAGTGGCGCGGCTACTTATCTCTACCAATTTTCTACSGAATYLYQFS
ORF40........A..C..............GG....C...........V.
7806spc34bATTTGAGGGACTAAATAATGGGATCGTATTCAATFEGLNNGIVFN
ORF41.........CGC...C................G....A......S
CT6spc2bAATCCCCCCGTCAGGGATTCTCCCACGGGTTTCAATIPPSGILPRVS
ORF61...A...........AG................T.......V.....
CT15spc12bACTCTCCTTGCGACTATAAGTATGTGGGCAAGTCTSPCDYKYVGKS
ORF25............................T.................
CT15spc13bTCTATCTGTTCAATACTATGCCTCTAGGAGCAGGGCAAGYLFNTMPLGAGQ
ORF20.....................................G.............
CT15spc14bTTGATACAGGTGCCTTCCTAGGCTGTTATCTDTGAFLGCY
ORF25.......G................................
  • a In each row, the spacer (top) and the corresponding putative proto-spacer (bottom) are shown.

  • b Reverse complementary sequences are shown.

  • c Identical nucleotides and amino acids are indicated by dots. Synonymous and conservative changes are in bold and italic letters, respectively. The seed region identified in the subtype I-E CRISPR of E. coli (43) is underlined.