Table 1

Comparison of literature-based growth and fermentation rates with xylose as a sole carbon sourcea

StrainStrain descriptionAerobic growth rate (h−1)Xylose consumption rate (g · g−1 h−1)Ethanol production rate (g · g−1 h−1)Ethanol yield (g · g−1)Reference
BY4741-S1APiromyces XI-expressing strain overexpressing tal1 and Δgre30.0010.0070.00320.46This study
BY4741-S1A1Piromyces XI mutant (xylA*1) strain overexpressing tal1 and Δgre30.0010.00820.00410.50This study
BY4741-S1A2Piromyces XI mutant (xylA*2) strain overexpressing tal1 and Δgre30.0030.01230.00600.49This study
BY4741-S1A3Piromyces XI mutant (xylA*3) strain overexpressing tal1 and Δgre30.0090.01260.00560.45This study
BY4741-S2A3KPiromyces XI mutant (xylA*3) strain overexpressing tal1, XKS1, and Δgre30.0610.0570.0240.42This study
CEN.PK-RWB 202Piromyces XI-expressing strain0.005NAbNANA19
CEN.PK-RWB202-AFXEvolved strain from CEN.PK-RWB 202 for 160 days in xylose medium0.180.3400.1400.4222
CEN.PK- RWB217Piromyces XI-expressing strain overexpressing XKS1, TKL1, RPE1, RKI1, and Δgre30.221.0600.4560.4320
CEN.PK- TMB3066Piromyces XI-expressing strain overexpressing XKS1, TKL1, RPE1, RKI1, HXT7, and Δgre30.020.0050.0020.4316
BWY10XylClostridium XI-expressing strain (evolved industrial strain in xylose medium)0.040.070.030.438
CEN.PK2-1C-TMB 3424XR/XDH-expressing strain overexpressing XKS1, GAL2, TKL1, RPE1, RKI1, and Δgre3NA0.060.0070.1237
  • a The fermentation performances of S. cerevisiae BY4741-S1A, -S1A1, -S1A2, -S1A3, and -S2A3 were compared with previously reported results. The strains developed here have 10- to 30-fold- and 3- to 10-fold-higher rates than previously reported engineered strains for xylose isomerase pathways and oxidoreductase pathways, respectively.

  • b NA, not available.