Table 3

Nodulation test on various leguminous plants and genotypic characterization by Southern hybridization of nodulation genes

Bradyrhizobium strainsNodulation ona:nod gene hybridizationb
A. americanaG. maxAr. hypogaeaV. radiataM. atropurpureumS. rostratanodAnodBnodC
Divergent nod-containing strains
    SUTN1-2+0+++0YLCLJL
    SUTN1-3+0+++0YCLJL
    SUTN1-4+0+++0YLCLJL
    SUTN1-6+0+++0YLCLJL
    SUTN1-8+0+++0YLCLJ
    SUTN1-12+0+++0JCLJL
    SUTN1-13+0+++0YLCLJL
    SUTN2-1+0+++0YLCLJ
    SUTN2-2+0+++0YLCLJL
    SUTN2-3+0+++0YLJJL
    SUTN2-4+0+++0YLCLJL
    SUTN4-3+0+++0YLCLJ
    SUTN5-6+0+++0YLCLJL
    DOA1+0+++0YLCLJL
    DOA2+0+++0YLCLJL
    DOA3+0+++0YLCLJL
    DOA4+0+++0YLCLJL
    DOA6+0+++0NCLJL
    DOA7+0+++0YLCLJL
    DOA8+0+++0NCLJL
    DOA9+0+++0NCLJL
    DOA10+0+++0YLCLJL
nod-containing strains
    SUTN1-7+0+++0JJJ
    SUTN1-9+0+++0JJJ
    SUTN3-1+0+++0JJJ
    SUTN4-1+0+++0JJJ
    SUTN5-1+0+++0JJJ
    SUTN5-3+0+++0JJJ
    SUTN5-5+0+++0JJJ
    SUTN6-1+0+++0JJJ
    SUTN6-2+0+++0JJJ
    SUTN7-1+0+++0JJJ
    SUTN7-2+0+++0JJJ
    SUTN8-1+0+++0JJJ
    SUTN8-2+0+++0JJJ
    SUTN9-1+0+++0JJJ
    SUTN9-2+0+++0JJJ
    SUTN9-3+0+++0JJJ
    SUTN9-4+0+++0JJJ
    SUTN9-5+0+++0JJJ
    USDA1100++++0JJJ
nod-independent strains
    BTAi100+000NNN
    ORS27800+000NNN
  • a +, positive with effective nodule; 0, negative.

  • b Southern blot hybridization signals are presented in Fig. S3 in the supplemental material. J, signal obtained from B. japonicum USDA110 under a stringent condition; YL, signal obtained from strain SUTN6-2 (99% 16S rRNA similarity to B. yuanmingense) under a low-stringency condition; CL, signal obtained from strain SUTN7-2 (99% 16S rRNA similarity to B. canariense) under a low-stringency condition; JL, signal obtained from B. japonicum USDA110 under a low-stringency condition and from strains SUTN6-2 and SUTN7-2 under a stringent condition; N, not detected.