Table 1

Sequences for quantitative PCR primers and probes used in this studya

GenotypePrimer or probe sequence (5′–3′)
Forward primerReverse primerProbe
FGL phycodnavirusTTTCATTTTTGCCGATGGATTTGTGCGTACAATTCGTCGTTCGCCAAGCCCCATATCAGGA
FGL cyanomyovirusGCACAGATCAGCACCAGTGTGGATTAGCAGGCAGACGAAGCACTGGCGCTACATCTGGATCGA
FGL circovirusCCATCCCACCATTTATTTGCGGGTCCATCTGGAACTGGTAGGCATTGGGAAAAAGCTCTCTTGC
FGL geminivirusTCCGAGGAGCAGAGTATCGTATGCTAATATCGGGCGAGTGTTCACCGTCCTTGCGGGCAT
CL phycodnavirusGCAGGCCGAACAGAAGATACAAGGCACTGCGACAGGTTATGGCGCTTCTCCAGCATACAGCA
CL cyanomyovirusACGGTATCAAGGCCAATGAGCGACCACCGAAGTAGAAGGATGTCCAACTGTTAGGTCAGTGGGGT
CL circovirusGGAAGTCAAGGGTTCGTCAATACCATTCTCGGGGATCAAGGCCGAGGTTATCTGGATCACCAGC
CL geminivirusGGAATGCACCTCCGATAAGAAATGTCGTACCGTTGGAAGCGCCTGTGTCTTCGTACGTAAGCTTCC
  • a The sequences for oligonucleotide standards are presented in Table S1 in the supplemental material. Primers were designed using Primer3, based on assembled contiguous sequences from the water columns and soils of Fayetteville Green Lake (FGL) and Cayuga Lake (CL).