Table 1

Environmental samples from the farm collected before and after removal of the infected herd studied by qPCR and cultivation

Location or sourceMatrixbM. avium subsp. paratuberculosis prevalence by time and method of analysisa
Spring (0 mo)Spring (12 mo)Autumn (18 mo)Spring (24 mo)Total no. of samples examined/no. of positive samples (% positive)
No. of samples examined/no. of positive samplesqPCRCNo. of samples examined/no. of positive samplesqPCRCNo. of samples examined/no. of positive samplesqPCRCNo. of samples examined/no. of positive samplesqPCRC
Shed from cattleCobweb3/3102–10501/0001/0001/11020
Organic substrate1/110203/310201/110200
    Subtotal4/44/32/11/111/9 (82)
Shed from calvesCobweb4/2103001/0000
Organic substrate3/2102–10301/00002/2102–1030
    Subtotal7/41/01/02/211/6 (55)
Mow and feed preparation roomCobweb03/210202/110001/000
Organic substrate002/2101–10200
    Subtotal03/24/31/08/5 (63)
Outside environmentWaste pit5/5103–10411/110201/110400
Silage02/0002/110102/000
Fecal01/0001/0000
Others002/0001/000
    Subtotal5/54/16/23/018/8 (44)
Total (% positive)16/13 (81)112/6 (50)013/6 (46)07/3 (43)048/28 (58)
Geometric meanc3.09 × 1032.98 × 1022.20 × 1025.86 × 102
  • a Examinations were done before removal of animals from the farm (0 months) or 12, 18 and 24 months after removal of animals from the farm. qPCR values are the numbers of M. avium subsp. paratuberculosis cells per g or per ml of sample. C, cultivation.

  • b Organic substrate, floor scraping, instruments, sediment, or scraping from watering troughs; fecal, fecal samples from wild animals (roe deer; Capreolus capreolus); others, vegetables and flushing.

  • c Geometric mean calculated according to absolute values.