Table 2

GFC and GFD qPCR assay performance characteristics based on 11 standard curves separately run over a 6-month period

AssayRange of values for:
SlopeInterceptEfficiencyar2ROQb (no. of copies)
GFC−4.06, −3.3434.55, 39.540.88, 1.000.990, 0.99620-2 × 105
GFD−3.51 −3.0232.52, 38.260.96, 1.070.993, 0.999200-2 × 105
  • a Defined as 10(1/−slope)/2.

  • b Range of quantification: plasmid concentration range that remained linear to CT values (r2 >0.98) in all 11 standard curves over the 6-month period.